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Reduction of facial wrinkles by hydrolyzed water-soluble egg membrane associated with reduction of free radical stress and support of matrix production by dermal fibroblasts

Gitte S Jensen,1 Bijal Shah,2 Robert Holtz,3Ashok Patel,4 Donald C Lo2

1NIS Labs, Klamath Falls, OR, 2Department of Neurobiology, Center for Drug Discovery, Duke University Medical Center, Durham, NC, 3BioInnovation Laboratories, Inc., Lakewood, CO, 4Centre Manufacturing LLC, Eden Prairie, MN, USA

Objective: The aim of this study was to evaluate the effects of water-soluble
egg membrane (WSEM) on wrinkle reduction in a clinical pilot study and to elucidate specific mechanisms of action using primary human immune and dermal cell-based bioassays.

Methods: To evaluate the effects of topical application of WSEM (8%) on human skin, an open-label 8-week study was performed involving 20 healthy females between the age of 45 years and 65 years. High-resolution photography and digital analysis were used to evaluate the wrinkle depth in the facial skin areas beside the eye (crow’s feet). WSEM was tested for total antioxidant capacity and effects on the formation of reactive oxygen species by human polymorphonuclear cells. Human keratinocytes (HaCaT cells) were used for quantitative polymerase chain reaction analysis of the antioxidant response element genes Nqo1GclmGclc, and Hmox1. Evaluation of effects on human primary dermal fibroblasts in vitro included cellular viability and production of the matrix components collagen and elastin.
Results: Topical use of a WSEM-containing facial cream for 8 weeks resulted in a significant reduction of wrinkle depth (P<0.05). WSEM contained antioxidants and reduced the formation of reactive oxygen species by inflammatory cells in vitro. Despite lack of a quantifiable effect on Nrf2, WSEM induced the gene expression of downstream Nqo1GclmGclc, and Hmox1 in human keratinocytes. Human dermal fibroblasts treated with WSEM produced more collagen and elastin than untreated cells or cells treated with dbcAMP control. The increase in collagen production was statistically significant (P<0.05).
Conclusion: The topical use of WSEM on facial skin significantly reduced the wrinkle depth. The underlying mechanisms of this effect may be related to protection from free radical damage at the cellular level and induction of several antioxidant response elements, combined with stimulation of human dermal fibroblasts to secrete high levels of matrix components.

Authors Jensen GS, Shah B, Holtz R, Patel A, Lo DC 

Received 4 May 2016

Accepted for publication 29 July 2016 

Published 14 October 2016 Volume 2016:9 Pages 357—366 


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Ram Prasad

Peer reviewer comments 3 

Editor who approved publication: Dr Jeffrey Weinberg


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Support of Joint Function, Range of Motion, and Physical Activity Levels by Consumption of a Water-Soluble Egg Membrane Hydrolyzate

Jensen Gitte S., Lenninger Miki R., Beaman Joni L., Taylor Robert, and Benson Kathleen F.. Journal of Medicinal Food. September 2015, 18(9): 1042-1048. doi:10.1089/jmf.2015.0041.


This study evaluated the effects of consumption of hydrolyzed water-soluble egg membrane (WSEM) on joint function in an otherwise healthy population experiencing chronic pain. A randomized, double-blind, placebo-controlled crossover study included two 4-week periods of placebo and WSEM consumption, separated by a 4-week washout period. Twenty-five study participants were randomized to either the “placebo-first” or “WSEM first” sequence in the crossover trial, and 22 participants completed the study requirements. Range of motion (ROM) was assessed using digital inclinometry for joints associated with vertical weight bearing from neck to knees and for shoulders. Pain at rest and when physically active was scored for the same anatomical areas using visual analog scales (VAS). Physical functioning was tracked using questionnaires with VAS. Consumption of WSEM was associated with improved ROM for neck, spine, hips, and knees, with ROM for the neck and right knee being significantly improved during WSEM consumption compared to placebo (P<.05). ROM improvement for the dominant shoulder was highly significant during WSEM consumption (P<.01). Physical activity levels were significantly higher after WSEM than after placebo consumption (P<.05). Many aspects of physical functioning as part of daily living improved. Subgroup analysis showed rapid improvement of lower back pain after 5 days of WSEM consumption compared to placebo consumption (P<.05) in subjects who participated in the study during the winter season. Daily consumption of 450mg WSEM was associated with improved joint function, comfort during daily activities, and increased physical activity.


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